Pub. Date : 1998 May 15
PMID : 9634001
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We examined the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on two transcription factors, CAAT/enhancer binding protein-alpha (C/EBPalpha) and beta (C/EBPbeta), involved in the coordination of gene expression in adipose and liver. | Polychlorinated Dibenzodioxins | CCAAT/enhancer binding protein (C/EBP), alpha | Mus musculus |
| 2 | We examined the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on two transcription factors, CAAT/enhancer binding protein-alpha (C/EBPalpha) and beta (C/EBPbeta), involved in the coordination of gene expression in adipose and liver. | Polychlorinated Dibenzodioxins | CCAAT/enhancer binding protein (C/EBP), alpha | Mus musculus |
| 3 | A single dose of TCDD (100 microg/kg) to male C57BL mice resulted in a time- and dose-dependent decrease in the level of C/EBPalpha mRNA in adipose tissue and liver, and a reciprocal increase in C/EBPbeta mRNA. | Polychlorinated Dibenzodioxins | CCAAT/enhancer binding protein (C/EBP), alpha | Mus musculus |
| 4 | Gel shift analysis using hepatic nuclear extracts from control and TCDD-treated mice and an oligonucleotide containing a C/EBP recognition element revealed a time-dependent change in DNA-protein complexes formed. | Polychlorinated Dibenzodioxins | CCAAT/enhancer binding protein (C/EBP), alpha | Mus musculus |
| 5 | Gel shift analysis using hepatic nuclear extracts from control and TCDD-treated mice and an oligonucleotide containing a C/EBP recognition element revealed a time-dependent change in DNA-protein complexes formed. | Oligonucleotides | CCAAT/enhancer binding protein (C/EBP), alpha | Mus musculus |
| 6 | Bands corresponding to C/EBPalpha, as determined by supershift analysis, diminished in TCDD-treated animals over a 7-day time period, whereas two new bands corresponding to C/EBPbeta, not present in control extracts, were increased significantly in treated samples. | Polychlorinated Dibenzodioxins | CCAAT/enhancer binding protein (C/EBP), alpha | Mus musculus |
| 7 | These results showed that TCDD caused rapid, reciprocal changes in C/EBPalpha and C/EBPbeta mRNAs and DNA binding in the adipose and liver of male C57BL mice and induced C/EBPbeta in hepatoma cells in an AhR-dependent manner. | Polychlorinated Dibenzodioxins | CCAAT/enhancer binding protein (C/EBP), alpha | Mus musculus |