Pub. Date : 1994 Jan 28
PMID : 8300592
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Irreversible inactivation of 5-lipoxygenase by leukotriene A4. | Leukotriene A4 | arachidonate 5-lipoxygenase | Homo sapiens |
| 2 | We report that leukotriene A4, the electrophilic product of 5-lipoxygenase catalysis, irreversibly inactivates the enzyme. | Leukotriene A4 | arachidonate 5-lipoxygenase | Homo sapiens |
| 3 | Leukotriene A4 inactivates cellular 5-lipoxygenase without inhibiting its translocation from the cytosol to the membrane, suggesting that it impairs catalysis without impairing formation of the complex between 5-lipoxygenase and its membrane-associated activating protein. | Leukotriene A4 | arachidonate 5-lipoxygenase | Homo sapiens |
| 4 | Leukotriene A4 inactivates cellular 5-lipoxygenase without inhibiting its translocation from the cytosol to the membrane, suggesting that it impairs catalysis without impairing formation of the complex between 5-lipoxygenase and its membrane-associated activating protein. | Leukotriene A4 | arachidonate 5-lipoxygenase | Homo sapiens |
| 5 | Consistent with this, leukotriene A4 inactivates purified 5-lipoxygenase from human neutrophils, via saturable, pseudo first-order kinetics with a rate constant, ki = 0.14 min-1 and a dissociation constant, Ki = 2.1 +/- 0.7 microM. | Leukotriene A4 | arachidonate 5-lipoxygenase | Homo sapiens |
| 6 | Preincubation with leukotriene A4 diminished the incorporation of radiolabeled material, consistent with irreversible modification of 5-lipoxygenase by its metastable product, leukotriene A4. | Leukotriene A4 | arachidonate 5-lipoxygenase | Homo sapiens |
| 7 | Preincubation with leukotriene A4 diminished the incorporation of radiolabeled material, consistent with irreversible modification of 5-lipoxygenase by its metastable product, leukotriene A4. | Leukotriene A4 | arachidonate 5-lipoxygenase | Homo sapiens |