Title : Agonist-induced expression of tissue inhibitor of metalloproteinases and metalloproteinases by human macrophages is regulated by endogenous prostaglandin E2 synthesis.

Pub. Date : 1995 Jan

PMID : 7798641






10 Functional Relationships(s)
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1 Agonist-induced expression of tissue inhibitor of metalloproteinases and metalloproteinases by human macrophages is regulated by endogenous prostaglandin E2 synthesis. Dinoprostone TIMP metallopeptidase inhibitor 1 Homo sapiens
2 The regulatory effect of endogenously synthesized eicosanoid metabolites on the expression of tissue inhibitor of metalloproteinases (TIMP), interstitial collagenase, and 92-kDa gelatinase by human macrophages was examined. Eicosanoids TIMP metallopeptidase inhibitor 1 Homo sapiens
3 The regulatory effect of endogenously synthesized eicosanoid metabolites on the expression of tissue inhibitor of metalloproteinases (TIMP), interstitial collagenase, and 92-kDa gelatinase by human macrophages was examined. Eicosanoids TIMP metallopeptidase inhibitor 1 Homo sapiens
4 Regardless of the agonist used, TIMP production by macrophages was inhibited 65% by indomethacin, synthesis of interstitial collagenase was reduced 70%, and expression of 92-kDa gelatinase was decreased 40%. Indomethacin TIMP metallopeptidase inhibitor 1 Homo sapiens
5 The agonist-stimulated increase in TIMP and collagenase production was directly correlated to the cumulative prostaglandin E2 level induced by the agonist used. Dinoprostone TIMP metallopeptidase inhibitor 1 Homo sapiens
6 The mechanism of indomethacin inhibition of TIMP and collagenase production was studied by labeling the cells with [35S]-methionine and performing immunoprecipitation using specific antiserum. Indomethacin TIMP metallopeptidase inhibitor 1 Homo sapiens
7 Indomethacin markedly inhibited the lipopolysaccharide-induced biosynthesis of both TIMP and collagenase. Indomethacin TIMP metallopeptidase inhibitor 1 Homo sapiens
8 Northern analysis revealed parallel suppression of TIMP and collagenase steady-state mRNA levels by indomethacin, indicating pretranslational control. Indomethacin TIMP metallopeptidase inhibitor 1 Homo sapiens
9 The regulation of inflammatory-cell TIMP and interstitial collagenase expression by prostaglandin E2 suggests that therapy inhibiting the cellular response to prostaglandins may be useful in cutaneous and systemic disease states involving macrophage-mediated connective-tissue destruction. Dinoprostone TIMP metallopeptidase inhibitor 1 Homo sapiens
10 The regulation of inflammatory-cell TIMP and interstitial collagenase expression by prostaglandin E2 suggests that therapy inhibiting the cellular response to prostaglandins may be useful in cutaneous and systemic disease states involving macrophage-mediated connective-tissue destruction. Prostaglandins TIMP metallopeptidase inhibitor 1 Homo sapiens