Pub. Date : 1984 Dec
PMID : 6239876
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Carbohydrate moiety of von Willebrand factor is not necessary for maintaining multimeric structure and ristocetin cofactor activity but protects from proteolytic degradation. | Carbohydrates | von Willebrand factor | Homo sapiens |
| 2 | To better define the role of carbohydrate in the structure and ristocetin cofactor activity of von Willebrand factor, we have removed up to 83% of total hexose by sequential treatment of the molecule with endo-beta-N-acetyl-glucosaminidase F (endo F), neuraminidase, and beta-galactosidase. | Carbohydrates | von Willebrand factor | Homo sapiens |
| 3 | All multimers of unreduced carbohydrate-modified von Willebrand factor migrated more rapidly in SDS-agarose, but the triplet pattern of individual multimers was unchanged. | Carbohydrates | von Willebrand factor | Homo sapiens |
| 4 | This suggested that carbohydrate was protecting von Willebrand factor against traces of one or more protease contaminants. | Carbohydrates | von Willebrand factor | Homo sapiens |
| 5 | In contrast, the multimeric structure of von Willebrand factor with intact carbohydrate was not affected by plasmin under similar conditions. | Carbohydrates | von Willebrand factor | Homo sapiens |
| 6 | These studies suggest that carbohydrate protects von Willebrand factor from disaggregation occurring secondarily to proteolytic attack but does not play a direct role in maintaining its multimeric structure or ristocetin cofactor activity. | Carbohydrates | von Willebrand factor | Homo sapiens |