Title : Click chemistry and optogenetic approaches to visualize and manipulate phosphatidic acid signaling.

Pub. Date : 2022 Apr

PMID : 35276134






4 Functional Relationships(s)
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1 First, we describe techniques to visualize PA production by phospholipase D (PLD) enzymes, which are major producers of PA, called Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation (IMPACT). Alcohols glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
2 First, we describe techniques to visualize PA production by phospholipase D (PLD) enzymes, which are major producers of PA, called Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation (IMPACT). Alcohols glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
3 First, we describe techniques to visualize PA production by phospholipase D (PLD) enzymes, which are major producers of PA, called Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation (IMPACT). Alcohols glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
4 IMPACT harnesses the ability of endogenous PLD enzymes to accept bioorthogonally tagged alcohols in transphosphatidylation reactions to generate functionalized reporter lipids that are subsequently fluorescently tagged via click chemistry. Alcohols glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens