Title : Oral administration of berberine limits post-traumatic osteoarthritis development and associated pain via AMP-activated protein kinase (AMPK) in mice.

Pub. Date : 2022 Jan

PMID : 34687898






7 Functional Relationships(s)
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1 Oral administration of berberine limits post-traumatic osteoarthritis development and associated pain via AMP-activated protein kinase (AMPK) in mice. Berberine protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens
2 OBJECTIVE: We investigated the effect of berberine, a natural plant product that can activate AMP-activated protein kinase (AMPK), on OA development and associated pain in mice. Berberine protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens
3 DESIGN: Human primary knee chondrocytes were utilized to investigate how AMPK is activated by berberine. Berberine protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens
4 RESULTS: Berberine induced phosphorylation of AMPKalpha (Thr172) via liver kinase B1 (LKB1), the major upstream kinase of AMPK, in chondrocytes in vitro. Berberine protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens
5 IHC analysis of WT DMM knee cartilage further revealed that berberine inhibited concomitant loss of expression and phosphorylation of AMPKalpha and expression of SIRT1 and SIRT3, suggesting an important role of activation of AMPK signaling in mediating beneficial effect of berberine. Berberine protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens
6 IHC analysis of WT DMM knee cartilage further revealed that berberine inhibited concomitant loss of expression and phosphorylation of AMPKalpha and expression of SIRT1 and SIRT3, suggesting an important role of activation of AMPK signaling in mediating beneficial effect of berberine. Berberine protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens
7 CONCLUSIONS: Berberine acts through AMPK to reduce joint structural damage and pain associated with post-traumatic OA in mice in vivo. Berberine protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens