Title : Spectrophotometric Determination of Glutamine Synthetase Activity in Cultured Cells.

Pub. Date : 2016 Oct 5

PMID : 31380466






1 Functional Relationships(s)
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1 The method for the assay of GS enzymatic activity relies on its gamma-glutamyl transferase reaction by measuring gamma-glutamylhydroxamate synthesized from glutamine and hydroxylamine, and the chromatographic separation of the reaction product from the reactants (Deuel et al., 1978). Hydroxylamine glutamate-ammonia ligase Homo sapiens