Pub. Date : 2018
PMID : 30517913
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Expression Levels of the Tyrosine Hydroxylase Gene and Histone Modifications Around its Promoter in the Locus Coeruleus and Ventral Tegmental Area of Rats during Forced Abstinence from Morphine. | Morphine | tyrosine hydroxylase | Rattus norvegicus |
| 2 | We studied whether morphine-induced changes in mRNA levels of the catecholamine biosynthesis enzyme, tyrosine hydroxylase (TH), are associated with histone modifications around the promoter of this gene in the locus coeruleus (LC) and ventral tegmental area (VTA) of rats. | Morphine | tyrosine hydroxylase | Rattus norvegicus |
| 3 | We studied whether morphine-induced changes in mRNA levels of the catecholamine biosynthesis enzyme, tyrosine hydroxylase (TH), are associated with histone modifications around the promoter of this gene in the locus coeruleus (LC) and ventral tegmental area (VTA) of rats. | Morphine | tyrosine hydroxylase | Rattus norvegicus |
| 4 | RESULTS: Analysis of our real-time quantitative reverse transcription PCR results by 1-way ANOVA showed significant upregulation (5.13 +- 0.39 folds) of LC levels of the TH transcript 24 h after the last injection of morphine to rats, when compared with 2 h and 7 days time points. | Morphine | tyrosine hydroxylase | Rattus norvegicus |
| 5 | Consistently, chromatin immunoprecipitation real-time quantitative PCR assays revealed that 24 h after the last injection of morphine, levels of H3 acetylation were significantly increased (4.12 +- 0.38 folds) at the promoter of the TH gene in the LC but not in the VTA. | Morphine | tyrosine hydroxylase | Rattus norvegicus |
| 6 | CONCLUSIONS: Results of the present study, for the first time, demonstrate the involvement of histone H3 acetylation in the regulation of TH gene expression in the LC of rats during forced abstinence from morphine. | Morphine | tyrosine hydroxylase | Rattus norvegicus |