Title : Label-free fluorescent assay of T4 polynucleotide kinase phosphatase activity based on G-quadruplexe-thioflavin T complex.

Pub. Date : 2017 Apr 1

PMID : 28153312






1 Functional Relationships(s)
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1 Upon the addition of T4 PNKP, the 3"-PO3 of the substrate was changed to 3"-OH which initiated the polymerization in the presence of terminal deoxynucleotidyl transferase and G-rich dNTP substrates. 3"-oh polynucleotide kinase 3'-phosphatase Homo sapiens