Pub. Date : 2016 Mar 11
PMID : 26797128
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Exposure to a high glucose concentration has recently been shown to increase phosphorylation of AMPK at Ser(485/491) of its alpha1/alpha2 subunit; however, the mechanism by which it does so is not known. | Serine | protein kinase AMP-activated catalytic subunit alpha 2 | Homo sapiens |
| 2 | We sought to determine whether PKC or PKD1 is involved in inhibition of AMPK by causing Ser(485/491) phosphorylation in skeletal muscle cells. | Serine | protein kinase AMP-activated catalytic subunit alpha 2 | Homo sapiens |
| 3 | This caused dose- and time-dependent increases in AMPK Ser(485/491) phosphorylation, which was associated with a ~60% decrease in AMPKalpha2 activity. | Serine | protein kinase AMP-activated catalytic subunit alpha 2 | Homo sapiens |
| 4 | This caused dose- and time-dependent increases in AMPK Ser(485/491) phosphorylation, which was associated with a ~60% decrease in AMPKalpha2 activity. | Serine | protein kinase AMP-activated catalytic subunit alpha 2 | Homo sapiens |
| 5 | Genetic knockdown of PKD1 also prevented Ser(485/491) phosphorylation of AMPK. | Serine | protein kinase AMP-activated catalytic subunit alpha 2 | Homo sapiens |
| 6 | Finally, recombinant PKD1 phosphorylated AMPKalpha2 at Ser(491) in cell-free conditions. | Serine | protein kinase AMP-activated catalytic subunit alpha 2 | Homo sapiens |
| 7 | These results identify PKD1 as a novel upstream kinase of AMPKalpha2 Ser(491) that plays a negative role in insulin signaling in muscle cells. | Serine | protein kinase AMP-activated catalytic subunit alpha 2 | Homo sapiens |