Title : S phase block following MEC1ATR inactivation occurs without severe dNTP depletion.

Pub. Date : 2015 Nov 24

PMID : 26603472






4 Functional Relationships(s)
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1 The S phase arrest is proposed to stem from a defect in Mec1-mediated degradation of Sml1, a conserved inhibitor of ribonucleotide reductase (RNR), causing a severe depletion in cellular dNTP pools. Parathion protein kinase MEC1 Saccharomyces cerevisiae S288C
2 Here, the casual link between the S phase arrest, Sml1, and dNTP-levels is examined using a temperature sensitive mec1 mutant. Parathion protein kinase MEC1 Saccharomyces cerevisiae S288C
3 The dNTP pool in mec1 is reduced by ~17% and GIS2 expression restores it, but only partially, to ~93% of a control. Parathion protein kinase MEC1 Saccharomyces cerevisiae S288C
4 Furthermore, unexpectedly modest effects of mec1 and GIS2 on dNTP levels suggest that the S phase arrest is unlikely to result from a severe depletion of dNTP pool as assumed, but a heightened sensitivity to small changes in its availability. Parathion protein kinase MEC1 Saccharomyces cerevisiae S288C