Title : Analytical isoelectric focusing with immobilized pH gradients of human apolipoprotein E from very low density lipoproteins and total plasma.

Pub. Date : 1990 Jan

PMID : 2313199






4 Functional Relationships(s)
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1 A method for analytical isoelectric focusing (IEF) of apolipoprotein E (apoE) in immobilized pH gradients (IPG) and immunodetection of the separated isoforms has been developed for use with either very low density lipoproteins (VLDL) or whole plasma. 2-Isopropoxyethanol apolipoprotein E Homo sapiens
2 Finally, the conditions for IPG of whole plasma using apoE monoclonal antibodies and enzyme-conjugated anti-mouse IgG for detection were established. 2-Isopropoxyethanol apolipoprotein E Homo sapiens
3 Thus, IPG focusing is shown to be a powerful method for resolution of the apoE sialoforms and apoE mutant forms. 2-Isopropoxyethanol apolipoprotein E Homo sapiens
4 Thus, IPG focusing is shown to be a powerful method for resolution of the apoE sialoforms and apoE mutant forms. 2-Isopropoxyethanol apolipoprotein E Homo sapiens