Pub. Date : 1990 Feb 20
PMID : 2110000
14 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The effects of GDP and of aurodox (N-methylkirromycin) on the affinity of elongation factor Tu (EF-Tu) for aminoacyl-tRNA (aa-tRNA) have been quantified spectroscopically by using Phe-tRNA(Phe)-Fl8, a functionally active analogue of Phe-tRNA(Phe) with a fluorescein dye convalently attached to the s4U-8 base. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 2 | The effects of GDP and of aurodox (N-methylkirromycin) on the affinity of elongation factor Tu (EF-Tu) for aminoacyl-tRNA (aa-tRNA) have been quantified spectroscopically by using Phe-tRNA(Phe)-Fl8, a functionally active analogue of Phe-tRNA(Phe) with a fluorescein dye convalently attached to the s4U-8 base. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 3 | The association of EF-Tu.GDP with Phe-tRNA(Phe)-Fl8 resulted in an average increase of 33% in fluorescein emission intensity. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 4 | This spectral change was used to monitor the extent of ternary complex formation as a function of EF-Tu.GDP concentration, and hence to obtain a dissociation constant, directly and at equilibrium, for the EF-Tu.GDP-containing ternary complex. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 5 | This spectral change was used to monitor the extent of ternary complex formation as a function of EF-Tu.GDP concentration, and hence to obtain a dissociation constant, directly and at equilibrium, for the EF-Tu.GDP-containing ternary complex. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 6 | This spectral change was used to monitor the extent of ternary complex formation as a function of EF-Tu.GDP concentration, and hence to obtain a dissociation constant, directly and at equilibrium, for the EF-Tu.GDP-containing ternary complex. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 7 | This spectral change was used to monitor the extent of ternary complex formation as a function of EF-Tu.GDP concentration, and hence to obtain a dissociation constant, directly and at equilibrium, for the EF-Tu.GDP-containing ternary complex. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 8 | The Kd for the Phe-tRNA(Phe)-Fl8.EF-Tu.GDP complex was found to average 28.5 microM, more than 33,000-fold greater than the Kd of the Phe-tRNA(Phe)-Fl8.EF-Tu.GTP complex under the same conditions. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 9 | The Kd for the Phe-tRNA(Phe)-Fl8.EF-Tu.GDP complex was found to average 28.5 microM, more than 33,000-fold greater than the Kd of the Phe-tRNA(Phe)-Fl8.EF-Tu.GTP complex under the same conditions. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 10 | Thus, the hydrolysis of the ternary complex GTP results in a dramatic decrease in the affinity of EF-Tu for aa-tRNA, thereby facilitating the release of EF-Tu.GDP from the aa-tRNA on the ribosome. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 11 | Thus, the hydrolysis of the ternary complex GTP results in a dramatic decrease in the affinity of EF-Tu for aa-tRNA, thereby facilitating the release of EF-Tu.GDP from the aa-tRNA on the ribosome. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 12 | The binding of aurodox to EF-Tu therefore both considerably strengthens EF-Tu.GDP affinity for aa-tRNA and also weakens EF-Tu.GTP affinity for aa-tRNA. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 13 | The binding of aurodox to EF-Tu therefore both considerably strengthens EF-Tu.GDP affinity for aa-tRNA and also weakens EF-Tu.GTP affinity for aa-tRNA. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |
| 14 | The binding of aurodox to EF-Tu therefore both considerably strengthens EF-Tu.GDP affinity for aa-tRNA and also weakens EF-Tu.GTP affinity for aa-tRNA. | Guanosine Diphosphate | eukaryotic translation elongation factor 1 alpha 1 | Homo sapiens |