PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
2110000-2	1990	The effects of GDP and of aurodox (N-methylkirromycin) on the affinity of elongation factor Tu (EF-Tu) for aminoacyl-tRNA (aa-tRNA) have been quantified spectroscopically by using Phe-tRNA(Phe)-Fl8, a functionally active analogue of Phe-tRNA(Phe) with a fluorescein dye convalently attached to the s4U-8 base.	Guanosine Diphosphate	15-18	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	74-94	
2110000-2	1990	The effects of GDP and of aurodox (N-methylkirromycin) on the affinity of elongation factor Tu (EF-Tu) for aminoacyl-tRNA (aa-tRNA) have been quantified spectroscopically by using Phe-tRNA(Phe)-Fl8, a functionally active analogue of Phe-tRNA(Phe) with a fluorescein dye convalently attached to the s4U-8 base.	Guanosine Diphosphate	15-18	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	96-101	
2110000-3	1990	The association of EF-Tu.GDP with Phe-tRNA(Phe)-Fl8 resulted in an average increase of 33% in fluorescein emission intensity.	Guanosine Diphosphate	25-28	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	19-24	
2110000-4	1990	This spectral change was used to monitor the extent of ternary complex formation as a function of EF-Tu.GDP concentration, and hence to obtain a dissociation constant, directly and at equilibrium, for the EF-Tu.GDP-containing ternary complex.	Guanosine Diphosphate	104-107	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	98-103	
2110000-4	1990	This spectral change was used to monitor the extent of ternary complex formation as a function of EF-Tu.GDP concentration, and hence to obtain a dissociation constant, directly and at equilibrium, for the EF-Tu.GDP-containing ternary complex.	Guanosine Diphosphate	104-107	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	205-210	
2110000-4	1990	This spectral change was used to monitor the extent of ternary complex formation as a function of EF-Tu.GDP concentration, and hence to obtain a dissociation constant, directly and at equilibrium, for the EF-Tu.GDP-containing ternary complex.	Guanosine Diphosphate	211-214	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	98-103	
2110000-4	1990	This spectral change was used to monitor the extent of ternary complex formation as a function of EF-Tu.GDP concentration, and hence to obtain a dissociation constant, directly and at equilibrium, for the EF-Tu.GDP-containing ternary complex.	Guanosine Diphosphate	211-214	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	205-210	
2110000-5	1990	The Kd for the Phe-tRNA(Phe)-Fl8.EF-Tu.GDP complex was found to average 28.5 microM, more than 33,000-fold greater than the Kd of the Phe-tRNA(Phe)-Fl8.EF-Tu.GTP complex under the same conditions.	Guanosine Diphosphate	39-42	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	33-38	
2110000-5	1990	The Kd for the Phe-tRNA(Phe)-Fl8.EF-Tu.GDP complex was found to average 28.5 microM, more than 33,000-fold greater than the Kd of the Phe-tRNA(Phe)-Fl8.EF-Tu.GTP complex under the same conditions.	Guanosine Diphosphate	39-42	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	152-157	
2110000-7	1990	Thus, the hydrolysis of the ternary complex GTP results in a dramatic decrease in the affinity of EF-Tu for aa-tRNA, thereby facilitating the release of EF-Tu.GDP from the aa-tRNA on the ribosome.	Guanosine Diphosphate	159-162	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	98-103	
2110000-7	1990	Thus, the hydrolysis of the ternary complex GTP results in a dramatic decrease in the affinity of EF-Tu for aa-tRNA, thereby facilitating the release of EF-Tu.GDP from the aa-tRNA on the ribosome.	Guanosine Diphosphate	159-162	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	153-158	
2110000-9	1990	The binding of aurodox to EF-Tu therefore both considerably strengthens EF-Tu.GDP affinity for aa-tRNA and also weakens EF-Tu.GTP affinity for aa-tRNA.	Guanosine Diphosphate	78-81	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	26-31	
2110000-9	1990	The binding of aurodox to EF-Tu therefore both considerably strengthens EF-Tu.GDP affinity for aa-tRNA and also weakens EF-Tu.GTP affinity for aa-tRNA.	Guanosine Diphosphate	78-81	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	72-77	
2110000-9	1990	The binding of aurodox to EF-Tu therefore both considerably strengthens EF-Tu.GDP affinity for aa-tRNA and also weakens EF-Tu.GTP affinity for aa-tRNA.	Guanosine Diphosphate	78-81	eukaryotic translation elongation factor 1 alpha 1	Homo sapiens	72-77