Title : Steady-state pHi, buffering power, and effect of CO2 in a smooth muscle-like cell line.

Pub. Date : 1990 Mar

PMID : 2107748






3 Functional Relationships(s)
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1 Steady-state pHi of BC3H-1 cells in NHB acidified reversibly on exposure to 0.5 mM 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS; 1.4 +/- 0.3 x 10(-4) pH/s), 1 mM amiloride (2.0 +/- 0.7 x 10(-4) pH/s), or Na-free solution (8.3 +/- 2.4 x 10(-4) pH/s) and alkalinized upon exposure to Cl-free solutions (9.7 +/- 2.2 x 10(-4) pH/s). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid glucose-6-phosphate isomerase 1 Mus musculus
2 Steady-state pHi of BC3H-1 cells in NHB acidified reversibly on exposure to 0.5 mM 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS; 1.4 +/- 0.3 x 10(-4) pH/s), 1 mM amiloride (2.0 +/- 0.7 x 10(-4) pH/s), or Na-free solution (8.3 +/- 2.4 x 10(-4) pH/s) and alkalinized upon exposure to Cl-free solutions (9.7 +/- 2.2 x 10(-4) pH/s). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid glucose-6-phosphate isomerase 1 Mus musculus
3 This new steady-state pHi acidified very slowly upon exposure to 1 mM amiloride (0.3 +/- 0.1 x 10(-4) pH/s), acidified more rapidly upon exposure to 0.5 mM DIDS (5.9 +/- 0.6 x 10(-4) pH/s) or Na-free solutions (9.8 +/- 1.0 x 10(-4) pH/s), and alkalinized on exposure to Cl-free solutions (24.5 +/- 1.3 x 10(-4) pH/s). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid glucose-6-phosphate isomerase 1 Mus musculus