Title : Persistent cAMP signaling by thyrotropin (TSH) receptors is not dependent on internalization.

Pub. Date : 2010 Oct

PMID : 20538910






1 Functional Relationships(s)
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1 We measured persistent TSHR signaling as an accumulation of cAMP in HEK-EM293 cells permanently expressing human TSHRs incubated with isobutylmethylxanthine for 30 min after washing the cells to remove unbound TSH, and TSHR internalization by fluorescence microscopy using Alexa-tagged TSH and binding assays using (125)I-TSH. Thyrotropin thyroid stimulating hormone receptor Homo sapiens