PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20538910-3 2010 We measured persistent TSHR signaling as an accumulation of cAMP in HEK-EM293 cells permanently expressing human TSHRs incubated with isobutylmethylxanthine for 30 min after washing the cells to remove unbound TSH, and TSHR internalization by fluorescence microscopy using Alexa-tagged TSH and binding assays using (125)I-TSH. Thyrotropin 23-26 thyroid stimulating hormone receptor Homo sapiens 113-117