Title : Glutamate-dependent active-site labeling of brain glutamate decarboxylase.

Pub. Date : 1990 Aug

PMID : 1973454






3 Functional Relationships(s)
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1 Analysis of labeled GAD by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis showed two labeled proteins with apparent molecular masses of 59 and 63 kDa. Sodium Dodecyl Sulfate glutamate-ammonia ligase Rattus norvegicus
2 Analysis of labeled GAD by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis showed two labeled proteins with apparent molecular masses of 59 and 63 kDa. Sodium Dodecyl Sulfate glutamate-ammonia ligase Rattus norvegicus
3 Three peaks of GAD activity (termed peaks I, II, and III) were separated by chromatography on phenyl-Sepharose, labeled with [32P]pyridoxal-P, purified by immunoaffinity chromatography, and analyzed by SDS-polyacrylamide gel electrophoresis. Sodium Dodecyl Sulfate glutamate-ammonia ligase Rattus norvegicus