Title : Real-time monitoring of phosphodiesterase inhibition in intact cells.

Pub. Date : 2008 Aug

PMID : 18467075






1 Functional Relationships(s)
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1 We used HEK 293 cells expressing genetic constructs encoding a PDE of interest (PDE3A, PDE4A1 or PDE5A) fused to cAMP and cGMP sensors, which allow to directly visualize changes in cyclic nucleotide concentrations in the vicinity of PDE molecules by fluorescence resonance energy transfer (FRET). Nucleotides, Cyclic phosphodiesterase 5A Homo sapiens