PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18467075-4 2008 We used HEK 293 cells expressing genetic constructs encoding a PDE of interest (PDE3A, PDE4A1 or PDE5A) fused to cAMP and cGMP sensors, which allow to directly visualize changes in cyclic nucleotide concentrations in the vicinity of PDE molecules by fluorescence resonance energy transfer (FRET). Nucleotides, Cyclic 181-198 phosphodiesterase 5A Homo sapiens 97-102