Pub. Date : 1991 Jul-Aug
PMID : 1791140
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Transcriptional changes in macrophage TNF-alpha expression following dimethylnitrosamine exposure in vivo. | Dimethylnitrosamine | tumor necrosis factor | Mus musculus |
| 2 | Since the production and expression of the macrophage-derived cytokine tumor necrosis factor-alpha (TNF-alpha) is associated with such anti-tumor activity, studies were performed to determine whether changes in TNF-alpha gene transcription and biosynthesis resulted following DMN exposure. | Dimethylnitrosamine | tumor necrosis factor | Mus musculus |
| 3 | Since the production and expression of the macrophage-derived cytokine tumor necrosis factor-alpha (TNF-alpha) is associated with such anti-tumor activity, studies were performed to determine whether changes in TNF-alpha gene transcription and biosynthesis resulted following DMN exposure. | Dimethylnitrosamine | tumor necrosis factor | Mus musculus |
| 4 | Since the production and expression of the macrophage-derived cytokine tumor necrosis factor-alpha (TNF-alpha) is associated with such anti-tumor activity, studies were performed to determine whether changes in TNF-alpha gene transcription and biosynthesis resulted following DMN exposure. | Dimethylnitrosamine | tumor necrosis factor | Mus musculus |
| 5 | Thioglycollate-elicited macrophages obtained from DMN-exposed animals displayed enhanced levels of constitutively expressed TNF-alpha transcripts compared to vehicle controls. | Dimethylnitrosamine | tumor necrosis factor | Mus musculus |
| 6 | Northern blot analysis of the time course expression of TNF-alpha following endotoxin (1 microgram/ml) stimulation in vitro showed a significantly greater induction of TNF-alpha transcripts in macrophages from DMN-exposed than control animals, with peak levels detected between 30 and 120 min. | Dimethylnitrosamine | tumor necrosis factor | Mus musculus |
| 7 | Northern blot analysis of the time course expression of TNF-alpha following endotoxin (1 microgram/ml) stimulation in vitro showed a significantly greater induction of TNF-alpha transcripts in macrophages from DMN-exposed than control animals, with peak levels detected between 30 and 120 min. | Dimethylnitrosamine | tumor necrosis factor | Mus musculus |
| 8 | However, stimulation of macrophages with IFN-gamma did greatly enhance the surface expression of membrane-bound TNF-alpha in cells from the DMN-treated animals. | Dimethylnitrosamine | tumor necrosis factor | Mus musculus |
| 9 | In media alone, a five-fold increase in TNF-alpha activity was observed at 6 h in supernatants from macrophage obtained from DMN-exposed animals compared to the vehicle group. | Dimethylnitrosamine | tumor necrosis factor | Mus musculus |