Pub. Date : 2006 Apr 14
PMID : 16452470
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Prolonged activation of cAMP-response element-binding protein and ATF-2 needed for nicotine-triggered elevation of tyrosine hydroxylase gene transcription in PC12 cells. | Nicotine | cAMP responsive element binding protein 1 | Rattus norvegicus |
| 2 | Phosphorylation (P-) of cAMP-response element-binding protein (CREB) by protein kinase A or mitogen-activated protein kinases was implicated in mediating the increased tyrosine hydroxylase (TH) gene expression after prolonged exposure to nicotine in vivo and in cell culture. | Nicotine | cAMP responsive element binding protein 1 | Rattus norvegicus |
| 3 | Phosphorylation (P-) of cAMP-response element-binding protein (CREB) by protein kinase A or mitogen-activated protein kinases was implicated in mediating the increased tyrosine hydroxylase (TH) gene expression after prolonged exposure to nicotine in vivo and in cell culture. | Nicotine | cAMP responsive element binding protein 1 | Rattus norvegicus |
| 4 | Treatment of PC12 cells with 200 microm nicotine triggered rapid but transient elevation of P-CREB followed by a second sustained rise after 2-5 h of continuous nicotine. | Nicotine | cAMP responsive element binding protein 1 | Rattus norvegicus |
| 5 | In contrast, protein kinase A inhibitor H-89 or Ca(2+)/calmodulin-activated protein kinase inhibitor KN-93 reduced the nicotine-triggered rise in P-CREB and TH promoter activity. | Nicotine | cAMP responsive element binding protein 1 | Rattus norvegicus |
| 6 | Knockdown of ATF-2 or CREB with siRNA did not alter basal TH promoter activity or mRNA but greatly attenuated the response to nicotine. | Nicotine | cAMP responsive element binding protein 1 | Rattus norvegicus |
| 7 | The results suggest that both ATF-2 and CREB mediate activation of TH gene transcription by nicotine. | Nicotine | cAMP responsive element binding protein 1 | Rattus norvegicus |