Pub. Date : 2003 Aug 1
PMID : 12907235
11 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Involvement of PKA and Sp1 in the induction of p27(Kip1) by tamoxifen. | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 2 | We have previously shown that tamoxifen (Tam) inhibits proliferation of estrogen receptor-negative human non-small cell lung cancer cells and this inhibition is associated with induction of p27(Kip1). | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 3 | We have previously shown that tamoxifen (Tam) inhibits proliferation of estrogen receptor-negative human non-small cell lung cancer cells and this inhibition is associated with induction of p27(Kip1). | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 4 | In this study, we investigated the mechanism by which Tam increases p27(Kip1) expression. | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 5 | Because intracellular p27(Kip1) protein level is mainly controlled via posttranslational regulation, we first tested whether Tam might affect protein stability of p27(Kip1). | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 6 | We next examined whether Tam enhanced p27(Kip1) expression through transcriptional activation. | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 7 | Our results demonstrated that Tam directly stimulated the p27(Kip1) promoter in lung cancer cells. | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 8 | Deletion and mutation analysis revealed that two Sp1 consensus sites located between -545 and -532bp from the transcription start site were crucial for the induction of p27(Kip1) by Tam. | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 9 | Our data also demonstrated that induction of p27(Kip1) by Tam was inhibited by protein kinase A inhibitor H89, but not by protein kinase C inhibitor calphostin C and mitogen-activated kinase kinase inhibitor PD98059. | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 10 | Taken together, our results suggest that Tam transcriptionally activates p27(Kip1) expression via the Sp1 consensus sites in the p27(Kip1) promoter and PKA is involved in this process. | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |
| 11 | Taken together, our results suggest that Tam transcriptionally activates p27(Kip1) expression via the Sp1 consensus sites in the p27(Kip1) promoter and PKA is involved in this process. | Tamoxifen | cyclin dependent kinase inhibitor 1B | Homo sapiens |