Pub. Date : 2000 May
PMID : 10804019
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We therefore investigated the role of hydrochlorothiazide (HCTZ) on OC production by the human osteoblast-like cell line MG-63. | Hydrochlorothiazide | bone gamma-carboxyglutamate protein | Homo sapiens |
| 2 | We therefore investigated the role of hydrochlorothiazide (HCTZ) on OC production by the human osteoblast-like cell line MG-63. | Hydrochlorothiazide | bone gamma-carboxyglutamate protein | Homo sapiens |
| 3 | HCTZ dose-dependently (1-100 microM) inhibited 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]-induced OC release by these cells (maximal effect, -40-50% and p < 0.005 by analysis of variance [ANOVA]) as measured by ELISA. | Hydrochlorothiazide | bone gamma-carboxyglutamate protein | Homo sapiens |
| 4 | This effect of HCTZ on OC release was caused by a direct effect on OC gene expression because Northern blot analysis revealed that OC messenger RNA (mRNA) levels were reduced in the presence of increasing doses of the diuretic (-47.2+/-4.0%; p < 0.0001 by paired ANOVA with 100 microM 13.6+/-0.49 pmol/mg protein/15 minutes; p < 0.05) in MG-63 cells. | Hydrochlorothiazide | bone gamma-carboxyglutamate protein | Homo sapiens |
| 5 | This effect of HCTZ on OC release was caused by a direct effect on OC gene expression because Northern blot analysis revealed that OC messenger RNA (mRNA) levels were reduced in the presence of increasing doses of the diuretic (-47.2+/-4.0%; p < 0.0001 by paired ANOVA with 100 microM 13.6+/-0.49 pmol/mg protein/15 minutes; p < 0.05) in MG-63 cells. | Hydrochlorothiazide | bone gamma-carboxyglutamate protein | Homo sapiens |
| 6 | This effect of HCTZ on OC release was caused by a direct effect on OC gene expression because Northern blot analysis revealed that OC messenger RNA (mRNA) levels were reduced in the presence of increasing doses of the diuretic (-47.2+/-4.0%; p < 0.0001 by paired ANOVA with 100 microM 13.6+/-0.49 pmol/mg protein/15 minutes; p < 0.05) in MG-63 cells. | Hydrochlorothiazide | bone gamma-carboxyglutamate protein | Homo sapiens |
| 7 | These studies indicate that HCTZ inhibits OC mRNA expression independently of an effect on VDR, YY1, or extracellular Ca2+ levels but involves changes in cFOS levels. | Hydrochlorothiazide | bone gamma-carboxyglutamate protein | Homo sapiens |
| 8 | As OC retards bone formation/mineralization, the inhibition of OC production by HCTZ could explain its preventive role in bone loss rate. | Hydrochlorothiazide | bone gamma-carboxyglutamate protein | Homo sapiens |