Title : Anti-inflammatory activities of hypocretenolides from Leontodon hispidus.

Pub. Date : 1999 Dec

PMID : 10630109






1 Functional Relationships(s)
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1 To obtain first information regarding the molecular targets which might be affected by this constituent, two in vitro bioassays were performed: (i) DNA binding activity of the transcription factor NF-kappa B was evaluated by electrophoretic mobility shift assay (EMSA) using TNF-alpha-activated Jurkat T cells and (ii) nitrite accumulation in cell culture supernatants of LPS-activated RAW 264.7 macrophages was determined as a parameter for inducible nitric oxide synthase (iNOS)-dependent nitric oxide release. Nitrites nuclear factor kappa B subunit 1 Homo sapiens