Title : Pyridyloxobutylation of guanine residues by 4-[(acetoxymethyl)nitrosamino]-1-(3-pyridyl)-1-butanone generates substrates of O6-alkylguanine-DNA alkyltransferase.

Pub. Date : 1996 Sep

PMID : 8870981






13 Functional Relationships(s)
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1 Pyridyloxobutylation of guanine residues by 4-[(acetoxymethyl)nitrosamino]-1-(3-pyridyl)-1-butanone generates substrates of O6-alkylguanine-DNA alkyltransferase. Guanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
2 Pyridyloxobutylation of guanine residues by 4-[(acetoxymethyl)nitrosamino]-1-(3-pyridyl)-1-butanone generates substrates of O6-alkylguanine-DNA alkyltransferase. 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone O-6-methylguanine-DNA methyltransferase Rattus norvegicus
3 Pyridyloxobutylation of DNA yields adducts that react with O6-alkylguanine-DNA alkyl-transferase (AGT) to prevent the repair of O6-methylguanine (O6-mG). O-(6)-methylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
4 Pyridyloxobutylation of DNA yields adducts that react with O6-alkylguanine-DNA alkyl-transferase (AGT) to prevent the repair of O6-methylguanine (O6-mG). O-(6)-methylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
5 Pyridyloxobutylation of DNA yields adducts that react with O6-alkylguanine-DNA alkyl-transferase (AGT) to prevent the repair of O6-methylguanine (O6-mG). O-(6)-methylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
6 Pyridyloxobutylation of DNA yields adducts that react with O6-alkylguanine-DNA alkyl-transferase (AGT) to prevent the repair of O6-methylguanine (O6-mG). O-(6)-methylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
7 The model pyridyloxobutylating agent, 4-[(acetoxymethyl)nitrosamino]-1-(3-pyridyl)-1-butanone (NNKOAc), was reacted with either poly(dAdT) or poly(dGdC) to generate DNA substrates for reaction with AGT. 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone O-6-methylguanine-DNA methyltransferase Rattus norvegicus
8 The model pyridyloxobutylating agent, 4-[(acetoxymethyl)nitrosamino]-1-(3-pyridyl)-1-butanone (NNKOAc), was reacted with either poly(dAdT) or poly(dGdC) to generate DNA substrates for reaction with AGT. 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone O-6-methylguanine-DNA methyltransferase Rattus norvegicus
9 Only the pyridyloxobutylated poly(dGdC) was able to prevent the ability of partially purified rat liver AGT to repair O6-mG. poly(dC-dG) O-6-methylguanine-DNA methyltransferase Rattus norvegicus
10 These results demonstrate that pyridyloxobutylation of only guanine residues produces adducts that react with AGT. Guanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
11 These AGT-reactive guanine adducts are relatively stable within DNA, with a half-life of 1-2 weeks at 37 degrees C. They represent up to 70% of the total HPB-releasing adducts in the NNKOAc-treated DNA. Guanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
12 These AGT-reactive guanine adducts are relatively stable within DNA, with a half-life of 1-2 weeks at 37 degrees C. They represent up to 70% of the total HPB-releasing adducts in the NNKOAc-treated DNA. 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone O-6-methylguanine-DNA methyltransferase Rattus norvegicus
13 We postulate that a potential AGT-reactive adduct is an O6-(pyridyloxobutyl)guanine adduct. o6-(pyridyloxobutyl)guanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus