Title : The phosphorylation and DNA binding of the DNA-binding domain of the orphan nuclear receptor NGFI-B.

Pub. Date : 1993 Nov 25

PMID : 8227042






6 Functional Relationships(s)
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1 The use of mutants in which either or both of these residues were replaced with alanines revealed that phosphorylation of Ser-350, located within the "A box," a motif necessary for DNA binding by NGFI-B, resulted in a decrease in binding to the NGFI-B response element, while phosphorylation of Ser-340 had little or no effect. Alanine nuclear receptor subfamily 4, group A, member 1 Rattus norvegicus
2 The use of mutants in which either or both of these residues were replaced with alanines revealed that phosphorylation of Ser-350, located within the "A box," a motif necessary for DNA binding by NGFI-B, resulted in a decrease in binding to the NGFI-B response element, while phosphorylation of Ser-340 had little or no effect. Alanine nuclear receptor subfamily 4, group A, member 1 Rattus norvegicus
3 The use of mutants in which either or both of these residues were replaced with alanines revealed that phosphorylation of Ser-350, located within the "A box," a motif necessary for DNA binding by NGFI-B, resulted in a decrease in binding to the NGFI-B response element, while phosphorylation of Ser-340 had little or no effect. Serine nuclear receptor subfamily 4, group A, member 1 Rattus norvegicus
4 The use of mutants in which either or both of these residues were replaced with alanines revealed that phosphorylation of Ser-350, located within the "A box," a motif necessary for DNA binding by NGFI-B, resulted in a decrease in binding to the NGFI-B response element, while phosphorylation of Ser-340 had little or no effect. Serine nuclear receptor subfamily 4, group A, member 1 Rattus norvegicus
5 The use of mutants in which either or both of these residues were replaced with alanines revealed that phosphorylation of Ser-350, located within the "A box," a motif necessary for DNA binding by NGFI-B, resulted in a decrease in binding to the NGFI-B response element, while phosphorylation of Ser-340 had little or no effect. Serine nuclear receptor subfamily 4, group A, member 1 Rattus norvegicus
6 The use of mutants in which either or both of these residues were replaced with alanines revealed that phosphorylation of Ser-350, located within the "A box," a motif necessary for DNA binding by NGFI-B, resulted in a decrease in binding to the NGFI-B response element, while phosphorylation of Ser-340 had little or no effect. Serine nuclear receptor subfamily 4, group A, member 1 Rattus norvegicus