Pub. Date : 1994 Apr
PMID : 8138587
10 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Induced expression of p52(PAI-1) in normal rat kidney cells by the microfilament-disrupting agent cytochalasin D. In established normal rat kidney (NRK) cells, synthesis of the 52 kDa type-1 inhibitor of plasminogen activator [p52(PAI-1)] is stimulated by the cell shape-modulating fungal metabolite cytochalasin D (CD). | Cytochalasin D | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |
| 2 | Induced expression of p52(PAI-1) in normal rat kidney cells by the microfilament-disrupting agent cytochalasin D. In established normal rat kidney (NRK) cells, synthesis of the 52 kDa type-1 inhibitor of plasminogen activator [p52(PAI-1)] is stimulated by the cell shape-modulating fungal metabolite cytochalasin D (CD). | Cytochalasin D | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |
| 3 | Induction paralleled the time course of morphologic change and reflected relatively specific increases in saponin-resistant p52(PAI-1) protein accumulation (approximating ten- to thirty-fold over control) and mRNA abundance (seven- to nine-fold). | Saponins | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |
| 4 | Sensitivity to tunicamycin indicated that N-linked post-translational modifications to this 43 kDa core species generated the full complement of 50 kDa (intermediate) and 52 kDa (mature) p52(PAI-1) glycosylated isoforms. | Tunicamycin | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |
| 5 | Sensitivity to tunicamycin indicated that N-linked post-translational modifications to this 43 kDa core species generated the full complement of 50 kDa (intermediate) and 52 kDa (mature) p52(PAI-1) glycosylated isoforms. | Nitrogen | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |
| 6 | CD-induced p52(PAI-1) expression occurred efficiently in quiescent NRK cells maintained under serum-free conditions as well as in fully serum-supplemented actively growing cultures. | Cytochalasin D | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |
| 7 | While 8-bromo-cAMP reduced both constitutive and transforming growth factor-beta-induced p52(PAI-1) synthesis by > 50%, no such inhibition was evident in short-term (4 h) CD-stimulated cultures. | 8-Bromo Cyclic Adenosine Monophosphate | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |
| 8 | Long-term (24 h) exposure of NRK/CD cells to 8-bromo-cAMP did result in an approximately 34% reduction in stimulated p52(PAI-1) expression, however, levels expressed by NRK/CD+cAMP populations remained markedly elevated relative to control values. | Cytochalasin D | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |
| 9 | Long-term (24 h) exposure of NRK/CD cells to 8-bromo-cAMP did result in an approximately 34% reduction in stimulated p52(PAI-1) expression, however, levels expressed by NRK/CD+cAMP populations remained markedly elevated relative to control values. | 8-Bromo Cyclic Adenosine Monophosphate | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |
| 10 | Long-term (24 h) exposure of NRK/CD cells to 8-bromo-cAMP did result in an approximately 34% reduction in stimulated p52(PAI-1) expression, however, levels expressed by NRK/CD+cAMP populations remained markedly elevated relative to control values. | Cyclic AMP | similar to Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP; P-52) | Rattus norvegicus |