Title : A hypoxia-responsive element mediates a novel pathway of activation of the inducible nitric oxide synthase promoter.

Pub. Date : 1995 Dec 1

PMID : 7500013






10 Functional Relationships(s)
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1 Picolinic acid, a catabolite of L-tryptophan, activates the transcription of the inducible nitric oxide synthase gene (iNOS) in IFN-gamma-treated murine macrophages. picolinic acid nitric oxide synthase 2, inducible Mus musculus
2 Picolinic acid, a catabolite of L-tryptophan, activates the transcription of the inducible nitric oxide synthase gene (iNOS) in IFN-gamma-treated murine macrophages. catabolite nitric oxide synthase 2, inducible Mus musculus
3 Picolinic acid, a catabolite of L-tryptophan, activates the transcription of the inducible nitric oxide synthase gene (iNOS) in IFN-gamma-treated murine macrophages. Tryptophan nitric oxide synthase 2, inducible Mus musculus
4 We performed functional studies on the 5" flanking region of the iNOS gene linked to a CAT reporter gene to identify the cis-acting element(s) responsible for the activation of iNOS transcription by picolinic acid. picolinic acid nitric oxide synthase 2, inducible Mus musculus
5 We performed functional studies on the 5" flanking region of the iNOS gene linked to a CAT reporter gene to identify the cis-acting element(s) responsible for the activation of iNOS transcription by picolinic acid. picolinic acid nitric oxide synthase 2, inducible Mus musculus
6 Transient transfection assays showed that the full-length iNOS promoter in the murine macrophage cell line ANA-1 was activated by the synergistic interaction between IFN-gamma and picolinic acid. picolinic acid nitric oxide synthase 2, inducible Mus musculus
7 Deletion or mutation of the iNOS promoter region from -227 to -209, containing a sequence homology to a hypoxia-responsive enhancer (iNOS-HRE), decreased picolinic acid- but not LPS-induced CAT activity by more than 70%. picolinic acid nitric oxide synthase 2, inducible Mus musculus
8 Deletion or mutation of the iNOS promoter region from -227 to -209, containing a sequence homology to a hypoxia-responsive enhancer (iNOS-HRE), decreased picolinic acid- but not LPS-induced CAT activity by more than 70%. picolinic acid nitric oxide synthase 2, inducible Mus musculus
9 Functional studies using a tk promoter-CAT reporter gene plasmid demonstrated that the iNOS-HRE was sufficient to confer inducibility by picolinic acid but not by IFN-gamma or LPS. picolinic acid nitric oxide synthase 2, inducible Mus musculus
10 Electrophoretic mobility shift assays confirmed that picolinic acid alone induced a specific binding activity to the iNOS-HRE. picolinic acid nitric oxide synthase 2, inducible Mus musculus