Title : A method for analyzing transcription using permeabilized cells.

Pub. Date : 1984 Dec

PMID : 6598004






2 Functional Relationships(s)
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1 The inhibition of UMP incorporation by 2 micrograms/ml alpha-amanitin was much greater at 23 degrees C than at 37 degrees C. The molecular weight of the RNA synthesized in permeabilized cells is broadly distributed with about 83% larger than 18 S. In vitro transcription of the mouse beta-major globin gene was studied by hybridizing 32P-labeled nascent RNA to filter-bound DNA sequences representing this gene and its flanking regions. Alpha-Amanitin hemoglobin, beta adult major chain Mus musculus
2 After induction by hexamethylene-bisacetamide, Friend cells exhibited more than fivefold increases in the rate of transcription for the beta-major globin gene as compared to the uninduced control cells. hexamethylene bisacetamide hemoglobin, beta adult major chain Mus musculus