Title : Evidence for mitochondrial localization of the hormone-responsive pool of Ca2+ in isolated hepatocytes.

Pub. Date : 1979 Sep 10

PMID : 381300






9 Functional Relationships(s)
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1 Conditions are described that allow chlortetracycline, a fluorescent probe of membrane-associated Ca2+, to monitor the content of the major exchangeable pool of intracellular Ca2+ present in the isolated rat hepatocyte. Chlortetracycline carbonic anhydrase 2 Rattus norvegicus
2 Conditions are described that allow chlortetracycline, a fluorescent probe of membrane-associated Ca2+, to monitor the content of the major exchangeable pool of intracellular Ca2+ present in the isolated rat hepatocyte. Chlortetracycline carbonic anhydrase 2 Rattus norvegicus
3 Chlortetracycline fluorescence is decreased in cells whose Ca2+ content is diminished by treatment either with carbonylcyanide-m-chlorophenylhydrazone or with ionophore A23187. Chlortetracycline carbonic anhydrase 2 Rattus norvegicus
4 Chlortetracycline fluorescence is decreased in cells whose Ca2+ content is diminished by treatment either with carbonylcyanide-m-chlorophenylhydrazone or with ionophore A23187. Carbonyl Cyanide m-Chlorophenyl Hydrazone carbonic anhydrase 2 Rattus norvegicus
5 Chlortetracycline fluorescence is decreased in cells whose Ca2+ content is diminished by treatment either with carbonylcyanide-m-chlorophenylhydrazone or with ionophore A23187. Calcimycin carbonic anhydrase 2 Rattus norvegicus
6 Norepinephrine releases Ca2+ from this exchangeable pool and decreases both the fluorescence signal and its subsequent response to A23187. Norepinephrine carbonic anhydrase 2 Rattus norvegicus
7 Norepinephrine releases Ca2+ from this exchangeable pool and decreases both the fluorescence signal and its subsequent response to A23187. Calcimycin carbonic anhydrase 2 Rattus norvegicus
8 Identification of the hormone-responsive pool of Ca2+ with the mitochondria is strengthened by comparison of the total calcium content of mitochondria isolated from control and hormone-treated animals. Calcium carbonic anhydrase 2 Rattus norvegicus
9 The uptake and release of Ca2+ in control and hormone-treated hepatocytes rendered permeable by treatment with digitonin is also consistent with this interpretation. Digitonin carbonic anhydrase 2 Rattus norvegicus