Title : Analysis of 1- and 3-Phosphohistidine (pHis) Protein Modification Using Model Enzymes Expressed in Bacteria.

Pub. Date : 2020

PMID : 31707652






2 Functional Relationships(s)
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1 Here, we describe reliable procedures for affinity purification, cofactor-binding analysis and antibody-based detection of phosphohistidine (pHis), on the putative human His kinases NME1 (NDPK-A) and NME2 (NDPK-B) and the glycolytic phosphoglycerate mutase PGAM1. 2-phosphoglycerate NME/NM23 nucleoside diphosphate kinase 1 Homo sapiens
2 Here, we describe reliable procedures for affinity purification, cofactor-binding analysis and antibody-based detection of phosphohistidine (pHis), on the putative human His kinases NME1 (NDPK-A) and NME2 (NDPK-B) and the glycolytic phosphoglycerate mutase PGAM1. 2-phosphoglycerate NME/NM23 nucleoside diphosphate kinase 2 Homo sapiens