Title : Porcine Circovirus Type 2 Induces ORF3-Independent Mitochondrial Apoptosis via PERK Activation and Elevation of Cytosolic Calcium.

Pub. Date : 2019 Apr 1

PMID : 30651358






5 Functional Relationships(s)
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1 We found that DeltaORF3-induced apoptosis was significantly reduced when the infected cells were treated with the selective PERK blocker GSK2606414 (GSK) or the general ER stress attenuator 4-phenylbutyrate (4-PBA). 7-methyl-5-(1-((3-(trifluoromethyl)phenyl)acetyl)-2,3-dihydro-1H-indol-5-yl)-7H-pyrrolo(2,3-d)pyrimidin-4-amine ankyrin repeat, SAM and basic leucine zipper domain containing 1 Homo sapiens
2 We found that DeltaORF3-induced apoptosis was significantly reduced when the infected cells were treated with the selective PERK blocker GSK2606414 (GSK) or the general ER stress attenuator 4-phenylbutyrate (4-PBA). 4-phenylbutyric acid ankyrin repeat, SAM and basic leucine zipper domain containing 1 Homo sapiens
3 We found that DeltaORF3-induced apoptosis was significantly reduced when the infected cells were treated with the selective PERK blocker GSK2606414 (GSK) or the general ER stress attenuator 4-phenylbutyrate (4-PBA). 4-phenylbutyric acid ankyrin repeat, SAM and basic leucine zipper domain containing 1 Homo sapiens
4 We propose that PCV2-driven ER stress is Cap dependent and could lead to mitochondrial apoptotic responses independent of ORF3 via perturbation of intracellular Ca2+ homeostasis and accumulation of ROS.IMPORTANCE PCV2 encodes protein ORF3, a putative protein with proapoptotic activity. Reactive Oxygen Species ankyrin repeat, SAM and basic leucine zipper domain containing 1 Homo sapiens
5 Here we clearly show that PCV2 infection or Cap expression induces ORF3-independent apoptosis via increased cytosolic and mitochondrial Ca2+ levels and cellular ROS levels as a result of activation of the PERK pathway. Reactive Oxygen Species ankyrin repeat, SAM and basic leucine zipper domain containing 1 Homo sapiens