Title : Human Chitotriosidase Is an Endo-Processive Enzyme.

Pub. Date : 2017

PMID : 28129403






4 Functional Relationships(s)
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Sentence
Compound Name
Protein Name
Organism
1 The catalytic domain of HCHT is connected to the carbohydrate binding module (CBM) through a flexible hinge region. Carbohydrates solute carrier family 5 member 7 Homo sapiens
2 Although the studies on soluble chitin derivatives suggest the endo-character of HCHT, the mode of action of the enzyme on crystalline chitin is not known. Chitin solute carrier family 5 member 7 Homo sapiens
3 Here, we made a thorough characterization of HCHT in terms of the mode of action, processivity, binding, and rate constants for the catalysis and dissociation using alpha-chitin as substrate. alpha-chitin solute carrier family 5 member 7 Homo sapiens
4 HCHT efficiently released the end-label from reducing-end labelled chitin and had also high probability (95%) of endo-mode initiation of processive run. Chitin solute carrier family 5 member 7 Homo sapiens