Pub. Date : 2015 Nov
PMID : 25737132
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | An optimum expression was induced by IPTG at a concentration of 0.2 mM for 8 h at 37 C. Glutathione-Superflow Resin affinity chromatography yielded the purified GST-rTRAIL protein which was confirmed by Western blot using anti-GST mouse monoclonal antibody. | Isopropyl Thiogalactoside | TNF superfamily member 10 | Rattus norvegicus |
| 2 | An optimum expression was induced by IPTG at a concentration of 0.2 mM for 8 h at 37 C. Glutathione-Superflow Resin affinity chromatography yielded the purified GST-rTRAIL protein which was confirmed by Western blot using anti-GST mouse monoclonal antibody. | Glutathione | TNF superfamily member 10 | Rattus norvegicus |
| 3 | The optimum prokaryotic cell expression of the human GST-rTRAIL was obtained by 0.2 mM IPTG induction for 8 h at 37 C. The denatured inclusion body protein can be refolded by dilution and dialysis and purified by Glutathione-Superflow Resin affinity chromatography. | Isopropyl Thiogalactoside | TNF superfamily member 10 | Rattus norvegicus |
| 4 | The optimum prokaryotic cell expression of the human GST-rTRAIL was obtained by 0.2 mM IPTG induction for 8 h at 37 C. The denatured inclusion body protein can be refolded by dilution and dialysis and purified by Glutathione-Superflow Resin affinity chromatography. | Glutathione | TNF superfamily member 10 | Rattus norvegicus |