Pub. Date : 1975 Mar
PMID : 242127
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | [Optimized determination and properties of NADPH-dependent glutathione reductase in serum. | NADP | glutathione-disulfide reductase | Homo sapiens |
| 2 | Imidazole was found to be the most suitable buffer material; the highest glutathione reductase activity in serum was always obtained with imidazole/HCl buffer, which, in contrast to all other tested buffers, also resulted in the maximal enzyme activity without preincubation. | imidazole | glutathione-disulfide reductase | Homo sapiens |
| 3 | Imidazole was found to be the most suitable buffer material; the highest glutathione reductase activity in serum was always obtained with imidazole/HCl buffer, which, in contrast to all other tested buffers, also resulted in the maximal enzyme activity without preincubation. | imidazole/hcl | glutathione-disulfide reductase | Homo sapiens |
| 4 | In imidazole buffer, the pH-activity curve of serum glutathione reductase shows a broad optimum between pH 6.5 and 6.9. | imidazole | glutathione-disulfide reductase | Homo sapiens |
| 5 | In the optimized assay system the glutathione reductase in the serum reacts specifically with GSSG and NADPH. | Glutathione Disulfide | glutathione-disulfide reductase | Homo sapiens |
| 6 | In the optimized assay system the glutathione reductase in the serum reacts specifically with GSSG and NADPH. | NADP | glutathione-disulfide reductase | Homo sapiens |