Pub. Date : 1990 May 15
PMID : 2159816
11 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We have studied the expression of the EPA/TIMP gene at the mRNA and protein levels during 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced megakaryoblastic differentiation of K562 human chronic myeloid leukemia cells. | Tetradecanoylphorbol Acetate | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 2 | We have studied the expression of the EPA/TIMP gene at the mRNA and protein levels during 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced megakaryoblastic differentiation of K562 human chronic myeloid leukemia cells. | Tetradecanoylphorbol Acetate | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 3 | Northern hybridization analysis showed that the EPA/TIMP mRNA was increased within 3 hours of TPA-induction and reached maximal levels (about 50-fold induction) during the first day of treatment. | Tetradecanoylphorbol Acetate | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 4 | The increase of EPA/TIMP mRNA correlated with increased EPA/TIMP protein biosynthesis and secretion: the TPA-induced cells secreted substantially enhanced amounts of metabolically labeled proteins, of which EPA/TIMP represented up to 50% after the first day of treatment (over 100-fold induction). | Tetradecanoylphorbol Acetate | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 5 | The increase of EPA/TIMP mRNA correlated with increased EPA/TIMP protein biosynthesis and secretion: the TPA-induced cells secreted substantially enhanced amounts of metabolically labeled proteins, of which EPA/TIMP represented up to 50% after the first day of treatment (over 100-fold induction). | Tetradecanoylphorbol Acetate | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 6 | The increase of EPA/TIMP mRNA correlated with increased EPA/TIMP protein biosynthesis and secretion: the TPA-induced cells secreted substantially enhanced amounts of metabolically labeled proteins, of which EPA/TIMP represented up to 50% after the first day of treatment (over 100-fold induction). | Tetradecanoylphorbol Acetate | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 7 | EPA/TIMP induction required continuous protein synthesis, being completely inhibited by addition of the protein synthesis inhibitor cycloheximide simultaneously with TPA, but only partially inhibited in a time-dependent manner if cycloheximide was added after TPA. | Cycloheximide | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 8 | EPA/TIMP induction required continuous protein synthesis, being completely inhibited by addition of the protein synthesis inhibitor cycloheximide simultaneously with TPA, but only partially inhibited in a time-dependent manner if cycloheximide was added after TPA. | Tetradecanoylphorbol Acetate | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 9 | EPA/TIMP induction required continuous protein synthesis, being completely inhibited by addition of the protein synthesis inhibitor cycloheximide simultaneously with TPA, but only partially inhibited in a time-dependent manner if cycloheximide was added after TPA. | Cycloheximide | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 10 | EPA/TIMP induction required continuous protein synthesis, being completely inhibited by addition of the protein synthesis inhibitor cycloheximide simultaneously with TPA, but only partially inhibited in a time-dependent manner if cycloheximide was added after TPA. | Tetradecanoylphorbol Acetate | TIMP metallopeptidase inhibitor 1 | Homo sapiens |
| 11 | This response was associated with enhanced activity of a transfected recombinant reporter plasmid containing binding sites for the jun/fos transcription factor complex (AP-1) similar to the TPA-responsive element (TRE) sequence we found in the EPA/TIMP gene promoter. | Tetradecanoylphorbol Acetate | TIMP metallopeptidase inhibitor 1 | Homo sapiens |