Title : Affinity labeling of lysine-149 in the anion-binding exosite of human alpha-thrombin with an N alpha-(dinitrofluorobenzyl)hirudin C-terminal peptide.

Pub. Date : 1990 Jul 10

PMID : 2119800






1 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 DNFB-[35S]Hir54-64 was reacted at a 10-fold molar excess with human alpha-thrombin in phosphate-buffered saline at pH 7.4 and 23 degrees C for 18 h. Autoradiographs of the product in reducing SDS-polyacrylamide gels revealed a single 35S-labeled band of Mr approximately 32,500. Phosphate-Buffered Saline coagulation factor II, thrombin Homo sapiens