Pub. Date : 2008 Jan
PMID : 18097593
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Subtoxic concentration of 8-Cl-Ado sensitizes human hepatoma cells to tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-triggered apoptosis. | 8-chloroadenosine | TNF superfamily member 10 | Homo sapiens |
| 2 | Subtoxic concentration of 8-Cl-Ado sensitizes human hepatoma cells to tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-triggered apoptosis. | 8-chloroadenosine | TNF superfamily member 10 | Homo sapiens |
| 3 | However, the molecular mechanism by which TRAIL cytotoxicity is amplified by 8-Cl-Ado is unknown. | 8-chloroadenosine | TNF superfamily member 10 | Homo sapiens |
| 4 | In the present study, we demonstrated by Western blot and real-time PCR that 8-Cl-Ado selectively up-regulated death receptor 5 (DR5), but not death receptor 4 (DR4), at both protein and RNA levels in human hepatoma cell line BEL-7402. | 8-chloroadenosine | TNF receptor superfamily member 10b | Homo sapiens |
| 5 | In the present study, we demonstrated by Western blot and real-time PCR that 8-Cl-Ado selectively up-regulated death receptor 5 (DR5), but not death receptor 4 (DR4), at both protein and RNA levels in human hepatoma cell line BEL-7402. | 8-chloroadenosine | TNF receptor superfamily member 10b | Homo sapiens |
| 6 | Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) confirmed that 8-Cl-Ado treatment facilitated transcription factor Sp1 binding to its cis-element -198/-189 in the DR5 promoter, suggesting that Sp1 is at least one of the 8-Cl-Ado-responsive transcription factors. | 8-chloroadenosine | TNF receptor superfamily member 10b | Homo sapiens |
| 7 | These data allowed us to draw a conclusion that 8-Cl-Ado-enhanced DR5 expression is regulated by Sp1 binding to the -198/-189 cis-element in DR5 promoter without affecting NF-kappaB activity in the hepatoma cells. | 8-chloroadenosine | TNF receptor superfamily member 10b | Homo sapiens |
| 8 | These data allowed us to draw a conclusion that 8-Cl-Ado-enhanced DR5 expression is regulated by Sp1 binding to the -198/-189 cis-element in DR5 promoter without affecting NF-kappaB activity in the hepatoma cells. | 8-chloroadenosine | TNF receptor superfamily member 10b | Homo sapiens |