Pub. Date : 2007 Jan
PMID : 16908189
2 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | All three natively purified proteins have, within experimental error, the same peptidyl-prolyl isomerase (PPIase) activity (k(cat)/K(m) approximately 1 x 10(6)M(-1)s(-1)), and bind a natural inhibitor, rapamycin, with the same high affinity (K(d) approximately 6 nM). | Sirolimus | FKBP prolyl isomerase 1B | Homo sapiens |
| 2 | However, refolding of the protein containing the longer tag in vitro results in reduced PPIase activity (the k(cat)/K(m) was reduced from 1 x 10(6)M(-1)s(-1) to 0.81 x 10(6)M(-1)s(-1)) and a 6-fold affinity loss for rapamycin. | Sirolimus | FKBP prolyl isomerase 1B | Homo sapiens |