Title : The Arabidopsis phenylalanine ammonia lyase gene family: kinetic characterization of the four PAL isoforms.

Pub. Date : 2004 Jun

PMID : 15276452






2 Functional Relationships(s)
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1 In this study, recombinant native AtPAL1, 2, and 4 were demonstrated to be catalytically competent for l-phenylalanine deamination, whereas AtPAL3, obtained as a N-terminal His-tagged protein, was of very low activity and only detectable at high substrate concentrations. Phenylalanine PHE ammonia lyase 1 Arabidopsis thaliana
2 AtPAL1, 2 and 4 had similar K(m) values (64-71 microM) for l-Phe, with AtPAL2 apparently being slightly more catalytically efficacious due to decreased K(m) and higher k(cat) values, relative to the others. Phenylalanine PHE ammonia lyase 1 Arabidopsis thaliana