Title : Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane.

Pub. Date : 2004 Jul 2

PMID : 15090548






11 Functional Relationships(s)
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1 Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane. Choline solute carrier family 5 member 7 Homo sapiens
2 CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline transporter. Hemicholinium 3 solute carrier family 5 member 7 Homo sapiens
3 CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline transporter. Hemicholinium 3 solute carrier family 5 member 7 Homo sapiens
4 CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline transporter. Choline solute carrier family 5 member 7 Homo sapiens
5 We now report that Par-4 is a negative regulator of CHT1 choline uptake activity. Choline solute carrier family 5 member 7 Homo sapiens
6 Transfection of neural IMR-32 cells with human CHT1 conferred Na(+)-dependent, HC-3-sensitive choline uptake that was effectively inhibited by cotransfection of Par-4. Hemicholinium 3 solute carrier family 5 member 7 Homo sapiens
7 Transfection of neural IMR-32 cells with human CHT1 conferred Na(+)-dependent, HC-3-sensitive choline uptake that was effectively inhibited by cotransfection of Par-4. Choline solute carrier family 5 member 7 Homo sapiens
8 Kinetic and cell-surface biotinylation assays showed that Par-4 inhibited CHT1-mediated choline uptake by reducing CHT1 expression in the plasma membrane without significantly altering the affinity of CHT1 for choline or HC-3. Choline solute carrier family 5 member 7 Homo sapiens
9 Kinetic and cell-surface biotinylation assays showed that Par-4 inhibited CHT1-mediated choline uptake by reducing CHT1 expression in the plasma membrane without significantly altering the affinity of CHT1 for choline or HC-3. Choline solute carrier family 5 member 7 Homo sapiens
10 Kinetic and cell-surface biotinylation assays showed that Par-4 inhibited CHT1-mediated choline uptake by reducing CHT1 expression in the plasma membrane without significantly altering the affinity of CHT1 for choline or HC-3. Choline solute carrier family 5 member 7 Homo sapiens
11 These results suggest that Par-4 is directly involved in regulating choline uptake by interacting with CHT1 and by reducing its incorporation on the cell surface. Choline solute carrier family 5 member 7 Homo sapiens