Title : Kinetics of activation of the P4 promoter of pBR322 by the Escherichia coli cyclic AMP receptor protein.

Pub. Date : 1992 Nov 1

PMID : 1445251






2 Functional Relationships(s)
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1 The effect of the cyclic-AMP/CRP complex on the linear P4 promoter was to increase the initial binding (KB) of RNA polymerase to the promoter by about a factor of 10, but the rate of isomerization of closed to open complex (kf) was unaffected. Cyclic AMP catabolite gene activator protein Escherichia coli
2 One molecule of CRP per promoter was required for activation, and the concentration of cyclic AMP producing half-maximal stimulation was about 7-8 microM. Cyclic AMP catabolite gene activator protein Escherichia coli