Pub. Date : 2001
PMID : 11423724
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | High glucose decreases matrix metalloproteinase-2 activity in rat mesangial cells via transforming growth factor-beta1. | Glucose | matrix metallopeptidase 2 | Rattus norvegicus |
| 2 | We have previously demonstrated that 72 kDa type IV collagenase activity is decreased in the rat mesangial cells cultured in high glucose media [Diabetes 1995;44:929-935]. | Glucose | matrix metallopeptidase 2 | Rattus norvegicus |
| 3 | Incubation of primary cultures of rat mesangial cells for 5 days in 30 vs. 5 mM glucose resulted in a 3-fold increase in production of total TGF-beta1, a significant decrease in MMP-2 activity and immunoreactive MMP-2 levels, and an increase in TIMP-2 levels. | Glucose | matrix metallopeptidase 2 | Rattus norvegicus |
| 4 | Incubation of primary cultures of rat mesangial cells for 5 days in 30 vs. 5 mM glucose resulted in a 3-fold increase in production of total TGF-beta1, a significant decrease in MMP-2 activity and immunoreactive MMP-2 levels, and an increase in TIMP-2 levels. | Glucose | matrix metallopeptidase 2 | Rattus norvegicus |
| 5 | Addition of exogenous TGF-beta1 to mesangial cells incubated in 5 mM glucose replicated the high glucose effect by producing a significant decrease in MMP-2 levels with a concurrent increase in TIMP-2 levels. | Glucose | matrix metallopeptidase 2 | Rattus norvegicus |
| 6 | Addition of exogenous TGF-beta1 to mesangial cells incubated in 5 mM glucose replicated the high glucose effect by producing a significant decrease in MMP-2 levels with a concurrent increase in TIMP-2 levels. | Glucose | matrix metallopeptidase 2 | Rattus norvegicus |
| 7 | Furthermore, glucose-induced inhibition of MMP-2 activity was completely blocked by neutralization of TGF-beta1 with anti-TGF-beta1 antibody. | Glucose | matrix metallopeptidase 2 | Rattus norvegicus |
| 8 | We conclude that the decrease in MMP-2 activity induced by glucose loading is mediated via TGF-beta1. | Glucose | matrix metallopeptidase 2 | Rattus norvegicus |