Title : Characteristics of protein-kinase-C- and ADP-ribosylation-factor-stimulated phospholipase D activities in human embryonic kidney cells.

Pub. Date : 1997 Sep 1

PMID : 9346296






9 Functional Relationships(s)
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1 Phospholipase D (PLD) activity in human embryonic kidney (HEK) cells is stimulated by phorbol-ester-activated protein kinase C (PKC) and by membrane receptors, the latter apparently acting via the GTP-binding proteins, ADP-ribosylation factor (ARF) and Rho. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
2 Phospholipase D (PLD) activity in human embryonic kidney (HEK) cells is stimulated by phorbol-ester-activated protein kinase C (PKC) and by membrane receptors, the latter apparently acting via the GTP-binding proteins, ADP-ribosylation factor (ARF) and Rho. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
3 In the present study, performed in cell-free preparations, we have characterized and compared the regulation of HEK cell PLD activity by the stable GTP analogue, guanosine 5"-O-[gamma-thio]triphosphate (GTP[S]), and the phorbol ester, phorbol 12-myristate 13-acetate (PMA). Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
4 In the present study, performed in cell-free preparations, we have characterized and compared the regulation of HEK cell PLD activity by the stable GTP analogue, guanosine 5"-O-[gamma-thio]triphosphate (GTP[S]), and the phorbol ester, phorbol 12-myristate 13-acetate (PMA). Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
5 Neomycin, which is known to complex with the PLD cofactor, phosphatidylinositol 4,5-bisphosphate, decreased basal and GTP[S]- or PMA-stimulated PLD activities with similar sensitivity. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
6 Neomycin, which is known to complex with the PLD cofactor, phosphatidylinositol 4,5-bisphosphate, decreased basal and GTP[S]- or PMA-stimulated PLD activities with similar sensitivity. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
7 PLD stimulation by GTP[S], but not by PMA, was markedly reduced upon cytosol depletion and reconstituted by purified recombinant ARF1. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
8 potentiated the GTP[S]-stimulated PLD activity. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
9 The results indicate that phorbol ester stimulation of PLD activity in HEK cells apparently occurs by a phosphorylation-dependent mechanism involving membrane-associated PKC isozymes but not ARF proteins, the major targets of GTP[S]" action. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens