Pub. Date : 1997 Sep 1
PMID : 9346296
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | Phospholipase D (PLD) activity in human embryonic kidney (HEK) cells is stimulated by phorbol-ester-activated protein kinase C (PKC) and by membrane receptors, the latter apparently acting via the GTP-binding proteins, ADP-ribosylation factor (ARF) and Rho. | Guanosine Triphosphate | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
2 | Phospholipase D (PLD) activity in human embryonic kidney (HEK) cells is stimulated by phorbol-ester-activated protein kinase C (PKC) and by membrane receptors, the latter apparently acting via the GTP-binding proteins, ADP-ribosylation factor (ARF) and Rho. | Guanosine Triphosphate | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
3 | In the present study, performed in cell-free preparations, we have characterized and compared the regulation of HEK cell PLD activity by the stable GTP analogue, guanosine 5"-O-[gamma-thio]triphosphate (GTP[S]), and the phorbol ester, phorbol 12-myristate 13-acetate (PMA). | Guanosine Triphosphate | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
4 | In the present study, performed in cell-free preparations, we have characterized and compared the regulation of HEK cell PLD activity by the stable GTP analogue, guanosine 5"-O-[gamma-thio]triphosphate (GTP[S]), and the phorbol ester, phorbol 12-myristate 13-acetate (PMA). | Guanosine Triphosphate | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
5 | Neomycin, which is known to complex with the PLD cofactor, phosphatidylinositol 4,5-bisphosphate, decreased basal and GTP[S]- or PMA-stimulated PLD activities with similar sensitivity. | Guanosine Triphosphate | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
6 | Neomycin, which is known to complex with the PLD cofactor, phosphatidylinositol 4,5-bisphosphate, decreased basal and GTP[S]- or PMA-stimulated PLD activities with similar sensitivity. | Guanosine Triphosphate | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
7 | PLD stimulation by GTP[S], but not by PMA, was markedly reduced upon cytosol depletion and reconstituted by purified recombinant ARF1. | Guanosine Triphosphate | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
8 | potentiated the GTP[S]-stimulated PLD activity. | Guanosine Triphosphate | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
9 | The results indicate that phorbol ester stimulation of PLD activity in HEK cells apparently occurs by a phosphorylation-dependent mechanism involving membrane-associated PKC isozymes but not ARF proteins, the major targets of GTP[S]" action. | Guanosine Triphosphate | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |