Pub. Date : 1997 Mar 3
PMID : 9120391
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | To determine if GraB can enter the cell, we treated YAC-1 or HeLa cells with FITC-labeled GraB and measured intracellular fluorescence with a high sensitivity CCD camera and image analyzer. | Fluorescein-5-isothiocyanate | granzyme B | Mus musculus |
| 2 | Uptake was inhibited at low temperature (4 degrees C) and by pretreatment with metabolic inhibitors, NaF and DNP, or cytochalasin B, a drug that both blocks microfilament formation, and FITC-GraB remained on the cell membrane localized in patches. | Fluorescein-5-isothiocyanate | granzyme B | Mus musculus |
| 3 | With the simultaneous addition of perforin and FITC-GraB, no significant increase in cytoplasmic fluorescence was observed over that found in cells treated only with FITC-GraB. | Fluorescein-5-isothiocyanate | granzyme B | Mus musculus |
| 4 | However, FITC-GraB was now detected in the nucleus of apoptotic cells labeling apoptotic bodies and localized areas within and along the nuclear membrane. | Fluorescein-5-isothiocyanate | granzyme B | Mus musculus |