Title : Didecanoyl phosphatidylcholine is a superior substrate for assaying mammalian phospholipase D.

Pub. Date : 1996 Nov 1

PMID : 8920991






2 Functional Relationships(s)
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1 Furthermore, PLD activity in cultured cells can only be detected by the available assay techniques in the presence of guanosine 5"-[gamma-thio]-triphosphate (GTP[S]) and a cytosolic factor [usually ADP-ribosylation factor (Arf)]. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
2 C10-PC was superior to C16-PC by a factor of 2-28 depending on assay conditions and tissue, and it allowed the detection of GTP[S]-and Arf-stimulated PLD activity without addition of phosphatidylinositol 4,5-bisphosphate. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens