Title : Restoration of Clostridium difficile toxin-B-inhibited phospholipase D by phosphatidylinositol 4,5-bisphosphate.

Pub. Date : 1996 Sep 15

PMID : 8856074






4 Functional Relationships(s)
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1 Addition of MgATP to permeabilized HEK cells increased basal PLD activity and potentiated PLD stimulation by the stable GTP analogue, guanosine 5"-[gamma-thio]triphosphate (GTP[S]), concomitant with a large increase in PtdIns(4,5)P2. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
2 Addition of MgATP to permeabilized HEK cells increased basal PLD activity and potentiated PLD stimulation by the stable GTP analogue, guanosine 5"-[gamma-thio]triphosphate (GTP[S]), concomitant with a large increase in PtdIns(4,5)P2. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
3 On the other hand, neomycin, which binds to PtdIns(4,5)P2, inhibited basal and GTP[S]-stimulated PLD activities. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens
4 In membranes of toxin-B-treated cells, basal and GTP[S]-stimulated PLD activities were reduced, when measured with exogenous phosphatidylcholine as enzyme substrate. Guanosine Triphosphate glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens