Title : Development of an optimal protocol for antigen retrieval: a 'test battery' approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalin-fixed paraffin sections.

Pub. Date : 1996 Jul

PMID : 8774494






4 Functional Relationships(s)
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1 Development of an optimal protocol for antigen retrieval: a "test battery" approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalin-fixed paraffin sections. Paraffin RB transcriptional corepressor 1 Homo sapiens
2 The immunohistochemical detection of pRB expression in formalin-paraffin sections of human cancer has potential advantages of convenience, economy, and compatibility with routine surgical pathology practice. Paraffin RB transcriptional corepressor 1 Homo sapiens
3 In this study, the antigen retrieval (AR) method has been applied to the immunohistochemical detection of pRB in paraffin-embedded tissues and a "test battery" approach has been developed to identify the principal variables that result in the optimal AR protocol. Paraffin RB transcriptional corepressor 1 Homo sapiens
4 A consistent intensity of immunohistochemical staining for pRB was achieved using the identified optimal AR protocol on formalin-paraffin sections. Paraffin RB transcriptional corepressor 1 Homo sapiens