Pub. Date : 1996 Apr 16
PMID : 8664271
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | A stable mixed disulfide between thioredoxin reductase and its substrate, thioredoxin: preparation and characterization. | Disulfides | peroxiredoxin 5 | Homo sapiens |
| 2 | In order to provide evidence for the proposed conformational change, a complex between TrR and its substrate Tr involving a mixed disulfide between TrR and Tr was prepared. | Disulfides | peroxiredoxin 5 | Homo sapiens |
| 3 | In order to provide evidence for the proposed conformational change, a complex between TrR and its substrate Tr involving a mixed disulfide between TrR and Tr was prepared. | Disulfides | peroxiredoxin 5 | Homo sapiens |
| 4 | The redox active disulfide of TrR is composed of Cys135 and Cys138, and the redox active disulfide of Tr is made up of Cys32 and Cys35. | Disulfides | peroxiredoxin 5 | Homo sapiens |
| 5 | Reductive titrations show that approximately 1 equiv of sodium dithionite or NADPH is required to fully reduce C135S-C32S, and treatment with NH4Cl and DTT demonstrates that the mixed disulfide between Cys138 of TrR C135S and Cys35 of TrC32S that locks the structure in a conformation where FAD can be reduced by NADPH, but electrons cannot flow from FADH2 to the mixed disulfide bond. | Disulfides | peroxiredoxin 5 | Homo sapiens |